Murray State University
The In Vitro Binding Effectiveness of Selected Pathogens Using Phyllosilicate Clays
Institution
Murray State University
Faculty Advisor/ Mentor
Dale Barnett; Teal Shultz
Abstract
This study tests a new product containing Hydrated Aluminosilicates which is comparable to an available product containing Phyllosilicate Clays. Tests are conducted determining the adsorption properties of these products and their ability to bind pathogens including salmonella, clostridium, and E.coli. These pathogens are associated with entercolitis in horses, dogs, and humans. The Phyllosilicate Clays help decrease damage caused by pathogens by binding to the toxins they produce. It will be determined whether this new product is as effective as the currently available product or more effective at binding endotoxins associated with the Clostridium organism as well as Salmonella and E.coli. To test adsorption properties of these products bacterial testing against microbes, toxicology testing against toxins produced by the pathogens, virology testing in vitro using ELISA (Enzyme-Linked ImmunoSorbent Assay) and EAA (a chemiluminescent Endotoxin Activity Assay) tests are performed. Serial dilutions are made creating suspensions of the respective equine strain of bacteria. Specific portions of the products tested will then be weighed and added to the suspensions. These suspensions are plated and concentrations of bacteria read. Using the electron microscope each bacterium is observed to compare the products binding effectiveness. Each sample is tested for their toxin binding ability using the ELISA test kits. These tests will conclude whether the new product is comparable to the previous product containing Phyllosilicate Clays or if it surpasses the ability of the previous product to bind to the toxins that cause detrimental effects to the well being of the equine, canine, and human.
The In Vitro Binding Effectiveness of Selected Pathogens Using Phyllosilicate Clays
This study tests a new product containing Hydrated Aluminosilicates which is comparable to an available product containing Phyllosilicate Clays. Tests are conducted determining the adsorption properties of these products and their ability to bind pathogens including salmonella, clostridium, and E.coli. These pathogens are associated with entercolitis in horses, dogs, and humans. The Phyllosilicate Clays help decrease damage caused by pathogens by binding to the toxins they produce. It will be determined whether this new product is as effective as the currently available product or more effective at binding endotoxins associated with the Clostridium organism as well as Salmonella and E.coli. To test adsorption properties of these products bacterial testing against microbes, toxicology testing against toxins produced by the pathogens, virology testing in vitro using ELISA (Enzyme-Linked ImmunoSorbent Assay) and EAA (a chemiluminescent Endotoxin Activity Assay) tests are performed. Serial dilutions are made creating suspensions of the respective equine strain of bacteria. Specific portions of the products tested will then be weighed and added to the suspensions. These suspensions are plated and concentrations of bacteria read. Using the electron microscope each bacterium is observed to compare the products binding effectiveness. Each sample is tested for their toxin binding ability using the ELISA test kits. These tests will conclude whether the new product is comparable to the previous product containing Phyllosilicate Clays or if it surpasses the ability of the previous product to bind to the toxins that cause detrimental effects to the well being of the equine, canine, and human.