Murray State University

Poster Title

The Distribution of Gastric Renal H+-K+-ATPase in the Outer Medullary Collecting Duct of Hyperglycemic Wild Type Mice as Shown by Immunocytochemical Staining

Institution

Murray State University

Abstract

The regulation of acid-base balance is known as homeostasis, and the kidneys play an important role in maintaining this balance. Acid secretion (proton secretion) takes place in epithelial cells of the kidney and is vital for the reabsorption and regeneration of bicarbonate that is used in metabolic acid production. We are currently studying renal H+-K+-ATPase in the OMCD. The OMCD is very important because it is the last place in which the regulation of the acid-base balance can take place. H+-K+-ATPase is a transporter which requires ATP in order to function. Its purpose is to reabsorb potassium (K) and secrete protons (H+), so it therefore, provides a mechanism for K conservation, while at the same time decreasing the acidity of the body. Two luminal proton ATPases, H+-ATPase and H+-K+-ATPase (HKA) both of which are in the intercalated cells of the OMCD, play an important role in acid-base homeostasis. It has been shown by Nakamura that HKA alpha 1 (gastric H+-K+-ATPase) is the essential H+-K+-ATPase under normal conditions, and that HKA alpha 2 (colonic H+-K+-ATPase) is induced and mediates increased proton secretion under K-depleted conditions. We are analyzing the density and distribution of the active, functioning gastric H+-K+-ATPase in the OMCD via the method of immunocytochemical staining under hyper and hypoglycemic conditions. Our data has shown that under normal conditions, the gastric H+-K+-ATPase is scattered along the cytoplasmic side of the plasma membrane in a ring shape and it is also slightly polarized to the apical membrane of the intercalated cells of the OMCD.

This document is currently not available here.

Share

COinS
 

The Distribution of Gastric Renal H+-K+-ATPase in the Outer Medullary Collecting Duct of Hyperglycemic Wild Type Mice as Shown by Immunocytochemical Staining

The regulation of acid-base balance is known as homeostasis, and the kidneys play an important role in maintaining this balance. Acid secretion (proton secretion) takes place in epithelial cells of the kidney and is vital for the reabsorption and regeneration of bicarbonate that is used in metabolic acid production. We are currently studying renal H+-K+-ATPase in the OMCD. The OMCD is very important because it is the last place in which the regulation of the acid-base balance can take place. H+-K+-ATPase is a transporter which requires ATP in order to function. Its purpose is to reabsorb potassium (K) and secrete protons (H+), so it therefore, provides a mechanism for K conservation, while at the same time decreasing the acidity of the body. Two luminal proton ATPases, H+-ATPase and H+-K+-ATPase (HKA) both of which are in the intercalated cells of the OMCD, play an important role in acid-base homeostasis. It has been shown by Nakamura that HKA alpha 1 (gastric H+-K+-ATPase) is the essential H+-K+-ATPase under normal conditions, and that HKA alpha 2 (colonic H+-K+-ATPase) is induced and mediates increased proton secretion under K-depleted conditions. We are analyzing the density and distribution of the active, functioning gastric H+-K+-ATPase in the OMCD via the method of immunocytochemical staining under hyper and hypoglycemic conditions. Our data has shown that under normal conditions, the gastric H+-K+-ATPase is scattered along the cytoplasmic side of the plasma membrane in a ring shape and it is also slightly polarized to the apical membrane of the intercalated cells of the OMCD.