Eastern Kentucky University

Poster Title

Characterization of Enzyme Formulations for Electrochemical Sensors

Institution

Eastern Kentucky University

Abstract

Enzyme containing formulations for constructing electrochemical sensors have been studied. An application of these enzyme electrodes is the rapid measurement of blood alcohol concentration. In the present study, ultraviolet light and a benzophenone initiator were used to initiate the free radical polymerization of ethylene glycol diacrylate. Formulations with various levels of the initiator, enzyme, stabilizing sugar and monomer were tested. Patterns were formed and the amount of hydrogel and enzyme immobilized were determined by extraction and measurement of the photoinitiator. Scanning electron microscopy (SEM) was used to characterize the surface and edges of the hydrogel in terms of smoothness and uniformity. Overall, formulations produced well-defined photopatterned hydrogels that immobilized the enzyme.

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Characterization of Enzyme Formulations for Electrochemical Sensors

Enzyme containing formulations for constructing electrochemical sensors have been studied. An application of these enzyme electrodes is the rapid measurement of blood alcohol concentration. In the present study, ultraviolet light and a benzophenone initiator were used to initiate the free radical polymerization of ethylene glycol diacrylate. Formulations with various levels of the initiator, enzyme, stabilizing sugar and monomer were tested. Patterns were formed and the amount of hydrogel and enzyme immobilized were determined by extraction and measurement of the photoinitiator. Scanning electron microscopy (SEM) was used to characterize the surface and edges of the hydrogel in terms of smoothness and uniformity. Overall, formulations produced well-defined photopatterned hydrogels that immobilized the enzyme.