Eastern Kentucky University

Poster Title

Study 2 (Henderson): 3-Nitrotyrosine as an Oxidative Stress Indicator in Wistar Rats Involving Moderate Traumatic Brain Injuries for Proteomic Analysis

Institution

Eastern Kentucky University

Abstract

As a biomarker of nitrosative stress, elevated levels of 3-Nitrotyrosine (3-NT) signify the presence of oxidative stress and decreased levels of antioxidant enzymes. Since TBI is profoundly related to oxidative stress, 3-NT can be utilized as in vivo marker of oxidative nitric oxide damage following TBI. By using a moderate traumatic brain injury model with Wistar rats, it is hypothesized that formation of 3-NT as an intermediate will predict the involvement of protein nitration and oxidative stress in the brain. In this experiment, the levels of 3-NT were significantly elevated in TBI injured, saline treated rats compared to those who sustained an injury and were treated with the glutathione mimetic, GCEE, providing insight into the relationship between protein nitration and oxidative stress.

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Study 2 (Henderson): 3-Nitrotyrosine as an Oxidative Stress Indicator in Wistar Rats Involving Moderate Traumatic Brain Injuries for Proteomic Analysis

As a biomarker of nitrosative stress, elevated levels of 3-Nitrotyrosine (3-NT) signify the presence of oxidative stress and decreased levels of antioxidant enzymes. Since TBI is profoundly related to oxidative stress, 3-NT can be utilized as in vivo marker of oxidative nitric oxide damage following TBI. By using a moderate traumatic brain injury model with Wistar rats, it is hypothesized that formation of 3-NT as an intermediate will predict the involvement of protein nitration and oxidative stress in the brain. In this experiment, the levels of 3-NT were significantly elevated in TBI injured, saline treated rats compared to those who sustained an injury and were treated with the glutathione mimetic, GCEE, providing insight into the relationship between protein nitration and oxidative stress.