Eastern Kentucky University

Poster Title

Incidence of Antibiotic Resistant Genes near Wastewater Treatment Plants

Institution

Eastern Kentucky University

Abstract

The incidence of antibiotic resistance genes near wastewater treatment plants (WWTP) was assessed. Water and sediment samples were collected from streams near WWTPs associated with small communities in south-central Kentucky. Samples were collected downstream, at the effluent of, and upstream from nine different WWTPs. The DNA was isolated using MOBIO kits. Genes for antibiotic resistance were then amplified using polymerase chain reaction (PCR) and visualized using agarose electrophoresis. Evidence for the tetA, tetB, and tetX genes were found in several WWTP, and was found upstream, downstream, and at the effluent.

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Incidence of Antibiotic Resistant Genes near Wastewater Treatment Plants

The incidence of antibiotic resistance genes near wastewater treatment plants (WWTP) was assessed. Water and sediment samples were collected from streams near WWTPs associated with small communities in south-central Kentucky. Samples were collected downstream, at the effluent of, and upstream from nine different WWTPs. The DNA was isolated using MOBIO kits. Genes for antibiotic resistance were then amplified using polymerase chain reaction (PCR) and visualized using agarose electrophoresis. Evidence for the tetA, tetB, and tetX genes were found in several WWTP, and was found upstream, downstream, and at the effluent.