Poster Title

Interaction Between Synthetic Exosomes and the Primo Vascular System

Grade Level at Time of Presentation

Senior

Institution

University of Louisville

KY House District #

16

KY Senate District #

3

Department

Physiology Dept.

Abstract

Undergraduate Author: Jared Hall

Co-Authors: Dr. Phillip Bauer, Dr. Gustavo Perez-Abadia, and Dr. Claudio Maldonado

Faculty Mentor: Dr. Claudio Maldonado

Department: Department of Physiology, University of Louisville, Louisville, KY

Interaction Between Synthetic Exosomes and the Primo Vascular System

The Primo Vascular System (PVS) is a novel anatomical system discovered by Bong Han Kim, a Korean scientist, in the early 1960’s. The structure is composed of a systemic network of thread-like vessels and nodes. Previous studies have confirmed the presence of stem cells in Primo Nodes (PNs), however little is known regarding the physiological function of the PVS. A sub-type of the PVS has been found inside lymphatic and vascular vessels. A recent study reported visualizing intra-lymphatic Primo Vessels (IL-PVs) using Hollow Gold Nano spheres measuring 50-120nm in diameter. Our overall hypothesis is that the PVS collects exsomes from lymphatic fluid, channeling them towards PNs to facilitate their interaction with stem and immune cells in response to physiological stimuli to maintain homeostasis. The purpose of this study was to develop synthetic exosomes (SEs) with a membrane lipid composition similar to those released by PC-3 prostate cancer cells. The SEs were then stained with victoria blue and injected into the lumbar lymph nodes of a Sprague-Dawley Rat. Over time IL-PV’s and PNs were visualized by the contrast provided by the dye. SEs appeared to penetrate the IL-PV wall and PNs. In conclusion we were able to create SEs of the appropriate size and similar lipid composition of those derived from PC-3 cancer cells, and the SEs appear to penetrate IL-PVs and PNs based on the contrast provided by Victoria blue dye.

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Interaction Between Synthetic Exosomes and the Primo Vascular System

Undergraduate Author: Jared Hall

Co-Authors: Dr. Phillip Bauer, Dr. Gustavo Perez-Abadia, and Dr. Claudio Maldonado

Faculty Mentor: Dr. Claudio Maldonado

Department: Department of Physiology, University of Louisville, Louisville, KY

Interaction Between Synthetic Exosomes and the Primo Vascular System

The Primo Vascular System (PVS) is a novel anatomical system discovered by Bong Han Kim, a Korean scientist, in the early 1960’s. The structure is composed of a systemic network of thread-like vessels and nodes. Previous studies have confirmed the presence of stem cells in Primo Nodes (PNs), however little is known regarding the physiological function of the PVS. A sub-type of the PVS has been found inside lymphatic and vascular vessels. A recent study reported visualizing intra-lymphatic Primo Vessels (IL-PVs) using Hollow Gold Nano spheres measuring 50-120nm in diameter. Our overall hypothesis is that the PVS collects exsomes from lymphatic fluid, channeling them towards PNs to facilitate their interaction with stem and immune cells in response to physiological stimuli to maintain homeostasis. The purpose of this study was to develop synthetic exosomes (SEs) with a membrane lipid composition similar to those released by PC-3 prostate cancer cells. The SEs were then stained with victoria blue and injected into the lumbar lymph nodes of a Sprague-Dawley Rat. Over time IL-PV’s and PNs were visualized by the contrast provided by the dye. SEs appeared to penetrate the IL-PV wall and PNs. In conclusion we were able to create SEs of the appropriate size and similar lipid composition of those derived from PC-3 cancer cells, and the SEs appear to penetrate IL-PVs and PNs based on the contrast provided by Victoria blue dye.