Poster Title

Serum Testosterone, Total Adiponectin, and Vascular Adiponectin Receptor R1 during Maturation of the Rat

Grade Level at Time of Presentation

Senior

Institution

University of Louisville

KY House District #

67

KY Senate District #

24

Department

Biology

Abstract

SERUM TESTOSTERONE, TOTAL ADIPONECTIN, AND VASCULAR ADIPONECTIN RECEPTOR R1 DURING MATURATION OF THE RAT. Christina Seibert, Tess Quesenberry, Paige Hart and J.M. Steffen (Mentor).

Adiponectin, a protein hormone secreted from adipocytes, has been linked to obesity and diabetes as well as vascular co-pathologies such as hypertension and atherosclerosis. Secretion of adiponectin has been reported to be regulated by testosterone, and the focus of this work is to evaluate serum testosterone and adiponectin, as well as vascular adiponectin receptor 1 levels during maturation in male and female rats. Our previous work has verified similarities in circulating levels of adiponectin and sex differences between rats and humans, and the present work presents a more complex attempt to verify the usefulness of the rat as a model. Serum and blood vessels were isolated from 3 week, 6 week and 9 week old rats and stored frozen until utilized. Serum testosterone was determined by ELISA (Enzo), serum adiponectin was determined by ELISA (ALPCO), and receptor 1 concentrations determined by ELISA (NeoBiolab). Serum adiponectin concentrations did not differ between 3 week old male and female rats, but the levels of adiponectin had fallen by 80% (P

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Serum Testosterone, Total Adiponectin, and Vascular Adiponectin Receptor R1 during Maturation of the Rat

SERUM TESTOSTERONE, TOTAL ADIPONECTIN, AND VASCULAR ADIPONECTIN RECEPTOR R1 DURING MATURATION OF THE RAT. Christina Seibert, Tess Quesenberry, Paige Hart and J.M. Steffen (Mentor).

Adiponectin, a protein hormone secreted from adipocytes, has been linked to obesity and diabetes as well as vascular co-pathologies such as hypertension and atherosclerosis. Secretion of adiponectin has been reported to be regulated by testosterone, and the focus of this work is to evaluate serum testosterone and adiponectin, as well as vascular adiponectin receptor 1 levels during maturation in male and female rats. Our previous work has verified similarities in circulating levels of adiponectin and sex differences between rats and humans, and the present work presents a more complex attempt to verify the usefulness of the rat as a model. Serum and blood vessels were isolated from 3 week, 6 week and 9 week old rats and stored frozen until utilized. Serum testosterone was determined by ELISA (Enzo), serum adiponectin was determined by ELISA (ALPCO), and receptor 1 concentrations determined by ELISA (NeoBiolab). Serum adiponectin concentrations did not differ between 3 week old male and female rats, but the levels of adiponectin had fallen by 80% (P