Poster Title

Role of Human metapneumovirus Phosphoprotein domains in the viral lifecycle

Presenter Information

Connor VanMeterFollow

Grade Level at Time of Presentation

Senior

Institution

University of Kentucky

KY House District #

45

KY Senate District #

12

Department

Molecular & Cellular Biochemistry

Abstract

Human metapneumovirus (HMPV) causes severe respiratory disease, especially in infants, the elderly, and the immunocompromised. HMPV Phosphoprotein (P), which normally interacts with the viral polymerase for replication, has been shown to play roles in other parts of the viral life cycle. Here we have generated deletion mutants of P to investigate the roles of three different regions in protein-protein interactions (e.g., actin), localization, and membrane disruption. To visualize these interactions, we observed infected and transfected cells using confocal microscopy. Co-expression of N and tagged P variants suggest that the C-terminus plays a role in the formation of inclusion bodies, or regions for genome replication and transcription. These results will aid understanding of how HMPV spreads from cell-to-cell and discovery of novel therapeutic targets.

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Role of Human metapneumovirus Phosphoprotein domains in the viral lifecycle

Human metapneumovirus (HMPV) causes severe respiratory disease, especially in infants, the elderly, and the immunocompromised. HMPV Phosphoprotein (P), which normally interacts with the viral polymerase for replication, has been shown to play roles in other parts of the viral life cycle. Here we have generated deletion mutants of P to investigate the roles of three different regions in protein-protein interactions (e.g., actin), localization, and membrane disruption. To visualize these interactions, we observed infected and transfected cells using confocal microscopy. Co-expression of N and tagged P variants suggest that the C-terminus plays a role in the formation of inclusion bodies, or regions for genome replication and transcription. These results will aid understanding of how HMPV spreads from cell-to-cell and discovery of novel therapeutic targets.