NEXT-GENERATION CELL ORGANELLES: BIOCHEMICAL ANALYSIS OF AUBERGINE-TUDOR PROTEIN COMPLEX
Germ cells are referred to as the immortally totipotent stem cells, which develop into all possible cell types and tissues after fertilization and are responsible for perpetuating the species. Unique electron-dense structures of the germ cells are cytoplasmic non-membranous organelles, referred to as germ granules, which enrich germline specificRNAs and proteins crucial for germ cell development. Both Tudor (Tud) protein and its interacting partner PIWI protein Aubergine (Aub) are germ granule components required for germ cell formation during early embryogenesis in the fruit fly Drosophila. However, the assembly of the granules from individual components, such asTud and Aub, and their structures are still unclear. Here we provide biochemical evidence for interactions ofTud and Aub with motor proteins, RNA helicases, and glycolytic enzymes. My data also show that Aub can be expressed and post-translationally modified in baculovirus expression system and subsequently purified. Purified Aub can be associated with other purified granule proteins. These data indicate that germ granules contain multiple proteins assembled with aTud-Aub complex for post-transcriptional gene regulation during germline development.
Year manuscript completed
Year degree awarded
Master of Science
Jesse D. Jones College of Science, Engineering and Technology
Dissertation Committee Chair
Thesis - Murray State Access only
Zheng, Jimiao and Zheng, Jimiao, "NEXT-GENERATION CELL ORGANELLES: BIOCHEMICAL ANALYSIS OF AUBERGINE-TUDOR PROTEIN COMPLEX" (2017). Murray State Theses and Dissertations. 24.