Poster Title

Characterization of a Basement Membrane Associated Protein Encoding Gene in Drosophila melanogaster

Grade Level at Time of Presentation

Senior

Major

Biology

Minor

Chemistry

Institution

Western Kentucky University

KY House District #

2

KY Senate District #

32

Department

Biology

Abstract

Title: Characterization of a Basement Membrane Associated Protein Encoding Gene in Drosophila melanogaster

Authors: Aref Ranjbar, Mayank Kapadia, Ajay Srivastava, PhD(faculty member, mentor)

Basement Membranes (BM) are important for normal development and tumor progression. In order to get a better understanding of BM dynamics we identified genes that encoded BM interacting proteins. One such gene is predicted to be involved in vesicle-mediated transport in Drosophila melanogaster. Here we characterize this gene by utilizing molecular biology techniques like immunohistochemistry, RNA in situ hybridization, and Western blot analysis utilizing antibodies generated in the laboratory. Western blot analysis identified this protein to be ~30.8 Kilo Daltons in size. Anti-body staining indicates tissue and cell specific localization pattern for this protein. This pattern is similar to RNA in situ hybridization pattern observed in various tissues. Data related to this proteins’ involvement in vesicle-mediated transport will be presented.

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Characterization of a Basement Membrane Associated Protein Encoding Gene in Drosophila melanogaster

Title: Characterization of a Basement Membrane Associated Protein Encoding Gene in Drosophila melanogaster

Authors: Aref Ranjbar, Mayank Kapadia, Ajay Srivastava, PhD(faculty member, mentor)

Basement Membranes (BM) are important for normal development and tumor progression. In order to get a better understanding of BM dynamics we identified genes that encoded BM interacting proteins. One such gene is predicted to be involved in vesicle-mediated transport in Drosophila melanogaster. Here we characterize this gene by utilizing molecular biology techniques like immunohistochemistry, RNA in situ hybridization, and Western blot analysis utilizing antibodies generated in the laboratory. Western blot analysis identified this protein to be ~30.8 Kilo Daltons in size. Anti-body staining indicates tissue and cell specific localization pattern for this protein. This pattern is similar to RNA in situ hybridization pattern observed in various tissues. Data related to this proteins’ involvement in vesicle-mediated transport will be presented.