Surveillance of air microbial content in the campus of Kentucky State University
Grade Level at Time of Presentation
Senior
Major
Biology
Minor
Chemistry
Institution
Kentucky State University
KY House District #
CD8
KY Senate District #
CD8
Faculty Advisor/ Mentor
Alexander Lai
Department
Biology
Abstract
To determine if there is a pattern of microbial contamination in air in a confined space such as a campus setting, we have conducted a long-term longitudinal indoor air microbial quality survey. Surveyed areas include hallways, library, and restrooms. Sedimentation method was adopted for simplicity and reproducible. Nutrient agar plates were distributed at strategic areas, and environmental conditions such as ambient air temperature and humidity were recorded. Other factors such as the volume of traffic and/or occupancy were noted. After exposure for an hour, the plates were then incubated at 37 C for 24 h. The number of colonies were counted, and representative colonies were further sub-cultured and/or Gram-stained to determine the microbial species. Longitudinal data analysis and multifactorial analysis were used to determine if there were correlations between number of colony-forming unit (CFU) to parameters such as seasonality, traffic, and ambient temperature and humidity. Several statistically significant observations were noted. For example, during the winter of 2015-2016, the month of March had a higher microbial content, and as expected, a higher usage of the restroom resulted in higher CFU in the collecting plates. Further breakdowns and analysis of our results will be presented. A long-term goal is to determine if the pattern of microbial contamination correlates to environmental parameters such as temperature and humidity. An implicit goal is to determine if there is a correlation to student absenteeism due to respiratory tract infection.
Surveillance of air microbial content in the campus of Kentucky State University
To determine if there is a pattern of microbial contamination in air in a confined space such as a campus setting, we have conducted a long-term longitudinal indoor air microbial quality survey. Surveyed areas include hallways, library, and restrooms. Sedimentation method was adopted for simplicity and reproducible. Nutrient agar plates were distributed at strategic areas, and environmental conditions such as ambient air temperature and humidity were recorded. Other factors such as the volume of traffic and/or occupancy were noted. After exposure for an hour, the plates were then incubated at 37 C for 24 h. The number of colonies were counted, and representative colonies were further sub-cultured and/or Gram-stained to determine the microbial species. Longitudinal data analysis and multifactorial analysis were used to determine if there were correlations between number of colony-forming unit (CFU) to parameters such as seasonality, traffic, and ambient temperature and humidity. Several statistically significant observations were noted. For example, during the winter of 2015-2016, the month of March had a higher microbial content, and as expected, a higher usage of the restroom resulted in higher CFU in the collecting plates. Further breakdowns and analysis of our results will be presented. A long-term goal is to determine if the pattern of microbial contamination correlates to environmental parameters such as temperature and humidity. An implicit goal is to determine if there is a correlation to student absenteeism due to respiratory tract infection.