ORCA | General Poster Session

Detecting Algal Contamination in Angiosperm DNA Extractions

Taylor M. Horton
Dayle E. Saar, Murray State University

Abstract/Description

Abstract. In molecular-based studies, universal PCR primers are effective due to their wide range of applicability. Unfortunately, this type of non-specific amplification can also be a drawback when DNA contaminants are unknowingly amplified. The internal transcribed spacer regions between the nuclear genes for ribosomal RNA are flanked by highly conserved genes. PCR primers for these genes have been shown to complementary base-pair with organisms from three kingdoms: Protista, Fungi, and Plantae. Current DNA extraction methods extract all DNA within and on an organism, regardless of its origin. This research project focuses on developing procedures for detecting algal DNA contaminants in angiosperm DNA extractions. This technique will be helpful for studies that include wet-climate or aquatic plants, which are especially susceptible to algal contamination.

Key words: algal contamination, DNA extraction, angiosperm DNA, aquatic plants

This paper has been withdrawn.
 

Detecting Algal Contamination in Angiosperm DNA Extractions

Abstract. In molecular-based studies, universal PCR primers are effective due to their wide range of applicability. Unfortunately, this type of non-specific amplification can also be a drawback when DNA contaminants are unknowingly amplified. The internal transcribed spacer regions between the nuclear genes for ribosomal RNA are flanked by highly conserved genes. PCR primers for these genes have been shown to complementary base-pair with organisms from three kingdoms: Protista, Fungi, and Plantae. Current DNA extraction methods extract all DNA within and on an organism, regardless of its origin. This research project focuses on developing procedures for detecting algal DNA contaminants in angiosperm DNA extractions. This technique will be helpful for studies that include wet-climate or aquatic plants, which are especially susceptible to algal contamination.

Key words: algal contamination, DNA extraction, angiosperm DNA, aquatic plants