Honors College Senior Thesis Presentations
Characterization of Pancreatic Development in CRISPR/Cas9 Zebrafish Model
Academic Level at Time of Presentation
Senior
Major
Chemistry/Pre-medicine
Minor
Cell Biology
List all Project Mentors & Advisor(s)
Dr. Gary ZeRuth
Presentation Format
Oral Presentation - Murray State Access only
Abstract/Description
Gli-similar 3 (Glis3) has been implicated in several human pathologies including neonatal diabetes, congenital hypothyroidism, and polycystic kidney disease. Numerous genome-wide association studies (GWAS) have additionally identified Glis3 as a risk locus for the development of type 2 diabetes mellitus. To elucidate Glis3 gene function, a CRISPR-mediated knockout line of zebrafish was developed by deleting a segment of the Glis3 coding region resulting in a non-functional protein product. At select time points in development, the resulting embryos were analyzed via fluorescence imaging to determine the effect of the Glis3 knockout on embryonic pancreatic development.
Spring Scholars Week 2023 Event
Honors College Senior Thesis Presentations
Characterization of Pancreatic Development in CRISPR/Cas9 Zebrafish Model
Gli-similar 3 (Glis3) has been implicated in several human pathologies including neonatal diabetes, congenital hypothyroidism, and polycystic kidney disease. Numerous genome-wide association studies (GWAS) have additionally identified Glis3 as a risk locus for the development of type 2 diabetes mellitus. To elucidate Glis3 gene function, a CRISPR-mediated knockout line of zebrafish was developed by deleting a segment of the Glis3 coding region resulting in a non-functional protein product. At select time points in development, the resulting embryos were analyzed via fluorescence imaging to determine the effect of the Glis3 knockout on embryonic pancreatic development.