Academic Level at Time of Presentation

Senior

Major

Wildlife Conservation/Zoological Conservation

List all Project Mentors & Advisor(s)

Dr. Howard H. Whiteman, PhD

Presentation Format

Poster Presentation

Abstract/Description

Salamanders have been used in a variety of different experiments to  determine the health of various ecosystems. Biofluorescence was recently studied in  amphibians in a study done in 2020. Not much is known about all its functions,  more studies are needed to fully understand how it works and how it can be affected  by environmental factors. Learning more about the functions of salamander  biofluorescence could lead to a greater understanding of how pollutants and human  impacts can affect salamanders and the health of their ecosystems. This project  aims to focus on tiger salamander biofluorescence and how its distribution and  intensity is affected by the exposure of natural sunlight. Unmarked, 2nd year larval  salamanders were all be taken from a single diverse pond to control for any  differences among the ponds. The larvae were involved in a project that consisted of  three different light treatments being dark, dimmed, and light. Within these light  treatments there were also two time treatments that were six and twelve days.  Their biofluorescence was photographed before and after the time treatments with a  blue excitation light and a 500 nm long-pass filter. All images were quantified using  ImageJ. The results from this experiment support the idea that salamander  biofluorescence may be partly environmentally dependent and this could provide  useful information for future studies to further understand the functions of  amphibian biofluorescence. Furthermore, more extensive research into this topic  could provide a new indicator of ecosystem health for use in conservation.

Spring Scholars Week 2026

Sigma Xi Poster Competition

Share

COinS
 

The Effects of Sun Exposure on the Intensity and Distribution of Salamander Biofluorescence

Salamanders have been used in a variety of different experiments to  determine the health of various ecosystems. Biofluorescence was recently studied in  amphibians in a study done in 2020. Not much is known about all its functions,  more studies are needed to fully understand how it works and how it can be affected  by environmental factors. Learning more about the functions of salamander  biofluorescence could lead to a greater understanding of how pollutants and human  impacts can affect salamanders and the health of their ecosystems. This project  aims to focus on tiger salamander biofluorescence and how its distribution and  intensity is affected by the exposure of natural sunlight. Unmarked, 2nd year larval  salamanders were all be taken from a single diverse pond to control for any  differences among the ponds. The larvae were involved in a project that consisted of  three different light treatments being dark, dimmed, and light. Within these light  treatments there were also two time treatments that were six and twelve days.  Their biofluorescence was photographed before and after the time treatments with a  blue excitation light and a 500 nm long-pass filter. All images were quantified using  ImageJ. The results from this experiment support the idea that salamander  biofluorescence may be partly environmentally dependent and this could provide  useful information for future studies to further understand the functions of  amphibian biofluorescence. Furthermore, more extensive research into this topic  could provide a new indicator of ecosystem health for use in conservation.