Western Kentucky University
Development of Lolium multiflorum Cell Lines Capable of High Phosphate Accumulation
Institution
Western Kentucky University
Faculty Advisor/ Mentor
Shivendra V. Sahi; Nilesh C. Sharma
Abstract
Non-point source of phosphorus (P) pollution causes an environmental concern and thus remedial measures are being currently investigated. In the search for a suitable plant system for P phytoremediation, Lolium multiflorum cultivars (Marshall and Gulf ryegrass) were tested for their phosphate removal capacity in hydroponics and pots. These grasses accumulated > 2% (dry weight) P in their shoots from Penriched solution. When grown in pots containing P- contaminated soil, their P accumulation reached to near 1% (shoot dry weight) under a specific cultural condition. To further manipulate the P removal efficiency of these grasses, cell cultures were established using seed explants, on Murashige and Skoog medium supplemented with increasing concentrations (125-5000 mg/L) of KH2PO4. Callus developed and proliferated vigorously in presence of P at a concentration of 5000 mg/L. Plants will be regenerated from P-habituated callus or cell cultures and clones will be screened for the variation in P uptake and accumulation.
Development of Lolium multiflorum Cell Lines Capable of High Phosphate Accumulation
Non-point source of phosphorus (P) pollution causes an environmental concern and thus remedial measures are being currently investigated. In the search for a suitable plant system for P phytoremediation, Lolium multiflorum cultivars (Marshall and Gulf ryegrass) were tested for their phosphate removal capacity in hydroponics and pots. These grasses accumulated > 2% (dry weight) P in their shoots from Penriched solution. When grown in pots containing P- contaminated soil, their P accumulation reached to near 1% (shoot dry weight) under a specific cultural condition. To further manipulate the P removal efficiency of these grasses, cell cultures were established using seed explants, on Murashige and Skoog medium supplemented with increasing concentrations (125-5000 mg/L) of KH2PO4. Callus developed and proliferated vigorously in presence of P at a concentration of 5000 mg/L. Plants will be regenerated from P-habituated callus or cell cultures and clones will be screened for the variation in P uptake and accumulation.