Kentucky State University
Protection against Pesticides Induced DNA Damage by Sinigrin, a Bio-Active Compound, in Human Lymphocyte: Comet Assay
Institution
Kentucky State University
Faculty Advisor/ Mentor
Avinash Tope
Abstract
Chronic low level exposure to environmental pollutants such as synthetic pesticides has been implicated in many health conditions such as oxidative stress, leading to DNA damage, a cause behind many serious diseases such as cancer. The objectives of the present study were: (1) to evaluate if exposure to a mixture of carbaryl, endosulfan and esfenvalerate, the most widely used pesticides in KY, at low levels (5-20 µM), has the potential to cause oxidative stress induced DNA damage and to compare the potential with exposure to hydrogen peroxide(100 mM), using human lymphocytes; and (2) to ascertain the DNA protective capacity of sinigrin, (10-30 mM), a bio-active compound found in vegetables such as broccoli, cabbage, Brussels sprouts, against the DNA damage caused due to exposure to pesticides. Two principal strategies were evaluated; preincubation and co-incubation. In pre-incubation, the lymphocytes were incubated for one hour with various concentrations of sinigrin, followed with incubation with various concentrations of pesticide mixture. In the other set, lymphocytes were simultaneously exposed to both sinigrin and the pesticide mixture. DNA damage was evaluated by Single Cell Gel Electrophoresis (Comet Assay). DNA protective efficiency of sinigrin was found at concentrations at and above 20 mM against pesticides with co-incubation strategy.
Protection against Pesticides Induced DNA Damage by Sinigrin, a Bio-Active Compound, in Human Lymphocyte: Comet Assay
Chronic low level exposure to environmental pollutants such as synthetic pesticides has been implicated in many health conditions such as oxidative stress, leading to DNA damage, a cause behind many serious diseases such as cancer. The objectives of the present study were: (1) to evaluate if exposure to a mixture of carbaryl, endosulfan and esfenvalerate, the most widely used pesticides in KY, at low levels (5-20 µM), has the potential to cause oxidative stress induced DNA damage and to compare the potential with exposure to hydrogen peroxide(100 mM), using human lymphocytes; and (2) to ascertain the DNA protective capacity of sinigrin, (10-30 mM), a bio-active compound found in vegetables such as broccoli, cabbage, Brussels sprouts, against the DNA damage caused due to exposure to pesticides. Two principal strategies were evaluated; preincubation and co-incubation. In pre-incubation, the lymphocytes were incubated for one hour with various concentrations of sinigrin, followed with incubation with various concentrations of pesticide mixture. In the other set, lymphocytes were simultaneously exposed to both sinigrin and the pesticide mixture. DNA damage was evaluated by Single Cell Gel Electrophoresis (Comet Assay). DNA protective efficiency of sinigrin was found at concentrations at and above 20 mM against pesticides with co-incubation strategy.