Murray State University
Immunoprecipitation of Gastric H+K+ATPase Beta Subunit in Hyperglycemic Mice
Institution
Murray State University
Faculty Advisor/ Mentor
Suguru Nakamura
Abstract
Our studies currently focus on renal H+K+ATPase in the outer medullary collecting ducts (OMCD) of the mouse kidney. The OMCD is the last place in the kidney where acid-base balance, homeostatis, takes place. In this experiment we created a hyperglycemic mouse model by means of a intraperitoneal injection of streptozocin and measured the amount of H+-K+-ATPase beta subunit protein and compared that amount to the amount of H+-K+-ATPase beta subunit protein in a normal wildtype mouse. Our means of measuring the amount of H+K+ATPase beta subunit protein is by Immunoprecipitation. We demonstrated that hyperglycemic mice have increased amounts of gastric H+-K+-ATPase beta subunit levels in the intercalated cells of the OMCD when compared to normal wildtype mice.
Immunoprecipitation of Gastric H+K+ATPase Beta Subunit in Hyperglycemic Mice
Our studies currently focus on renal H+K+ATPase in the outer medullary collecting ducts (OMCD) of the mouse kidney. The OMCD is the last place in the kidney where acid-base balance, homeostatis, takes place. In this experiment we created a hyperglycemic mouse model by means of a intraperitoneal injection of streptozocin and measured the amount of H+-K+-ATPase beta subunit protein and compared that amount to the amount of H+-K+-ATPase beta subunit protein in a normal wildtype mouse. Our means of measuring the amount of H+K+ATPase beta subunit protein is by Immunoprecipitation. We demonstrated that hyperglycemic mice have increased amounts of gastric H+-K+-ATPase beta subunit levels in the intercalated cells of the OMCD when compared to normal wildtype mice.