Western Kentucky University
Genomic Comparison of C. scatologenes to M. thermoacetica
Institution
Western Kentucky University
Faculty Advisor/ Mentor
Claire Rinehart; Kinchel Doerner
Abstract
The genome of C. scatologenes was sequenced by high-throughput short read sequencing, and 10,096 contigs were returned. In order to determine the function of individual genes and the proteins produced, all of the contigs of C. scatologenes were aligned to a closely related reference genome, M. thermoacetica (NC_007644) using BLASTn. BLAST alignments with an E-value less than e-50 were considered significant matches. Contigs that were found to overlap protein coding genes were also compared at the amino acid level. Summary statistics on the number matching and non-matching contigs and reference genes are reported.
Genomic Comparison of C. scatologenes to M. thermoacetica
The genome of C. scatologenes was sequenced by high-throughput short read sequencing, and 10,096 contigs were returned. In order to determine the function of individual genes and the proteins produced, all of the contigs of C. scatologenes were aligned to a closely related reference genome, M. thermoacetica (NC_007644) using BLASTn. BLAST alignments with an E-value less than e-50 were considered significant matches. Contigs that were found to overlap protein coding genes were also compared at the amino acid level. Summary statistics on the number matching and non-matching contigs and reference genes are reported.