Western Kentucky University
Immune System Response to High Intensity Exercise: Study 1: (Bean) High Intensity Exercise Increases Cell Death of B-Lymphocytes (CD19) in Anaerobically Trained Individuals
Institution
Western Kentucky University
Faculty Advisor/ Mentor
James Navalta
Abstract
This study was developed to determine the B-lymphocyte response in terms of migration or apoptosis (cell death) with intense bouts of exercise. Anaerobically trained individuals (n=5) performed 5-minute treadmill runs using 48%, 74%, 96% of their VO2 max, with 5-minute rest bouts in between each run. Finally a run utilizing intensity at 110% of VO2 max was conducted to exhaustion. Blood samples for immune parameters were collected pre-testing, immediate post, 1-hour post exercise, and 24-hour post exercise. Samples were incubated with antibody markers for the B-cell subfraction (CD19), apoptosis (annexin V), and migration (CX3CR1). Data were analyzed using a one-way ANOVA. Supramaximal exercise increased cell death of Bcells compared to resting values (P=0.021). Annexin V positive cells were then significantly decreased at 1-hour post (P=0.004) and remained depressed at 24-hour post (P=0.003). No significant changes were noted for CD19 count (P=0.24) or percent migration (P=0.36). Recent studies have failed to observe increases in the cell death of overall lymphocytes, however the results of this investigation indicate that the B-cell subset is more susceptible compared to other lymphocyte subfractions. This finding may have transient implications for antibody production and humoral immunity following exercise.
Immune System Response to High Intensity Exercise: Study 1: (Bean) High Intensity Exercise Increases Cell Death of B-Lymphocytes (CD19) in Anaerobically Trained Individuals
This study was developed to determine the B-lymphocyte response in terms of migration or apoptosis (cell death) with intense bouts of exercise. Anaerobically trained individuals (n=5) performed 5-minute treadmill runs using 48%, 74%, 96% of their VO2 max, with 5-minute rest bouts in between each run. Finally a run utilizing intensity at 110% of VO2 max was conducted to exhaustion. Blood samples for immune parameters were collected pre-testing, immediate post, 1-hour post exercise, and 24-hour post exercise. Samples were incubated with antibody markers for the B-cell subfraction (CD19), apoptosis (annexin V), and migration (CX3CR1). Data were analyzed using a one-way ANOVA. Supramaximal exercise increased cell death of Bcells compared to resting values (P=0.021). Annexin V positive cells were then significantly decreased at 1-hour post (P=0.004) and remained depressed at 24-hour post (P=0.003). No significant changes were noted for CD19 count (P=0.24) or percent migration (P=0.36). Recent studies have failed to observe increases in the cell death of overall lymphocytes, however the results of this investigation indicate that the B-cell subset is more susceptible compared to other lymphocyte subfractions. This finding may have transient implications for antibody production and humoral immunity following exercise.