Western Kentucky University
Biosynthesis of Gold and Silver Nanoparticles Using Yucca filamentosa Plant Extract
Institution
Western Kentucky University
Faculty Advisor/ Mentor
Shivendra Sahi
Abstract
Little is known about the efficacies of whole plant extracts from taxonomically diverse plant species in synthesizing gold and silver nanoparticles (Au/AgNPs) [1]. Since AuNPs show maximum absorbance at or around 550 nm, plant extracts from various plant species supplemented with 10 mM KAuCl4 for up to 35 h were spectrophotometrically evaluated at this wavelength [2]. Among the species tested, Yucca filamentosa showed maximum absorbance. Whereas, Y.filamentosa extract supplemented with 10 mM AgNO3 showed maximum absorbance at 450-500 nm and temporal analysis revealed saturation after 24 h [3]. The study clearly revealed the potential for using spectrophotometric technique in identifying the plant species and duration of treatment that are conducive for the bioreduction of KAuCl4 and AgNO3 into AuNPs and AgNPs, respectively. Further, Y. filamentosa extract supplemented with KAuCl4 were incubated at various temperatures (4°C, 22°C, and 37°C) for different time intervals (15 mins to 35 h). Spectrophotometric analysis clearly showed that 37°C is favorable for the synthesis of AuNPs over an incubation period of 24-30 h. Transmission Electron Microscopy (TEM) confirmed the formation of monodisperse spherical AuNPs and AgNPs and they were further validated by Energy-dispersive spectroscopy [EDS]. The results empirically demonstrated the efficacy of Yucca extract for the “green synthesis” of AuNPs and AgNO3.
Biosynthesis of Gold and Silver Nanoparticles Using Yucca filamentosa Plant Extract
Little is known about the efficacies of whole plant extracts from taxonomically diverse plant species in synthesizing gold and silver nanoparticles (Au/AgNPs) [1]. Since AuNPs show maximum absorbance at or around 550 nm, plant extracts from various plant species supplemented with 10 mM KAuCl4 for up to 35 h were spectrophotometrically evaluated at this wavelength [2]. Among the species tested, Yucca filamentosa showed maximum absorbance. Whereas, Y.filamentosa extract supplemented with 10 mM AgNO3 showed maximum absorbance at 450-500 nm and temporal analysis revealed saturation after 24 h [3]. The study clearly revealed the potential for using spectrophotometric technique in identifying the plant species and duration of treatment that are conducive for the bioreduction of KAuCl4 and AgNO3 into AuNPs and AgNPs, respectively. Further, Y. filamentosa extract supplemented with KAuCl4 were incubated at various temperatures (4°C, 22°C, and 37°C) for different time intervals (15 mins to 35 h). Spectrophotometric analysis clearly showed that 37°C is favorable for the synthesis of AuNPs over an incubation period of 24-30 h. Transmission Electron Microscopy (TEM) confirmed the formation of monodisperse spherical AuNPs and AgNPs and they were further validated by Energy-dispersive spectroscopy [EDS]. The results empirically demonstrated the efficacy of Yucca extract for the “green synthesis” of AuNPs and AgNO3.