University of Louisville
Impact of Quercetin on miR-21, Cell Proliferation and Migration of Metastatic and NonMetastatic Prostate Cancer Cell Lines
Institution
University of Louisville
Faculty Advisor/ Mentor
La Creis Kidd
Abstract
The over-expression of oncogenic microRNAs (miRs) may be counteracted by chemopreventive agents. Quercetin inhibits aggressive PCA (cell invasion, migration, proliferation) in vitro and modulates the expression of selected miRs in vivo. However, it is not clear whether quercetin modulates miR-21 expression and cancer behavior in African-American derived PCA cell line (E006AA). We hypothesized quercetin would decrease cell proliferation, migration and miR-21 levels in a metastatic (PC-3) and non-metastatic PCA cells (E006AA). After E006AA and PC3 cell were plated and treated with quercetin, cellular proliferation at 24 and 48 hrs was assessed with Trypan Blue and BrDU assays. For the cell migration assay, treated and untreated cells were plated onto silicon cell culture plates with five 2mm plugs. After removing the plugs, the area was photographed and quantified at 8-16 hrs. MiRNA levels were determined by qRT-PCR. There was a modest decrease in cell proliferation for PC3 and E006AA cells treated with quercetin (12.5-75µM) compared to vehicle control under the Trypan Blue assay. These effects only persisted in PC3 cells treated with 23uM quercetin under the BrDu assay. Quercetin treatment (23-39.475µM) revealed a 23 and 14% reduction in cell migration at 48hrs in PC3 and E006AA cells. The impact of quercetin on cell proliferation and migration is not mediated through a reduction in miR-21-3p. The findings of our study may serve as a foundation for future studies that seek to identify and validate new treatment strategies for individuals susceptible to pre- and metastatic PCA. Research was supported by NCI grant R25-CA134283.
Impact of Quercetin on miR-21, Cell Proliferation and Migration of Metastatic and NonMetastatic Prostate Cancer Cell Lines
The over-expression of oncogenic microRNAs (miRs) may be counteracted by chemopreventive agents. Quercetin inhibits aggressive PCA (cell invasion, migration, proliferation) in vitro and modulates the expression of selected miRs in vivo. However, it is not clear whether quercetin modulates miR-21 expression and cancer behavior in African-American derived PCA cell line (E006AA). We hypothesized quercetin would decrease cell proliferation, migration and miR-21 levels in a metastatic (PC-3) and non-metastatic PCA cells (E006AA). After E006AA and PC3 cell were plated and treated with quercetin, cellular proliferation at 24 and 48 hrs was assessed with Trypan Blue and BrDU assays. For the cell migration assay, treated and untreated cells were plated onto silicon cell culture plates with five 2mm plugs. After removing the plugs, the area was photographed and quantified at 8-16 hrs. MiRNA levels were determined by qRT-PCR. There was a modest decrease in cell proliferation for PC3 and E006AA cells treated with quercetin (12.5-75µM) compared to vehicle control under the Trypan Blue assay. These effects only persisted in PC3 cells treated with 23uM quercetin under the BrDu assay. Quercetin treatment (23-39.475µM) revealed a 23 and 14% reduction in cell migration at 48hrs in PC3 and E006AA cells. The impact of quercetin on cell proliferation and migration is not mediated through a reduction in miR-21-3p. The findings of our study may serve as a foundation for future studies that seek to identify and validate new treatment strategies for individuals susceptible to pre- and metastatic PCA. Research was supported by NCI grant R25-CA134283.