Poster Title

Metal and antibiotic cross-resistance among bacteria isolated from the Kentucky River

Grade Level at Time of Presentation

Senior

Major

Biomedical Sciences

Minor

Chemistry

Institution

Eastern Kentucky University

KY House District #

83

KY Senate District #

16

Department

Department of Biological Sciences

Abstract

Little is known about metal and antibiotic cross resistance among bacteria in aquatic settings. Water samples were collected from six locations along the Kentucky River. Plate counting was used to quantify metal-resistant bacteria. Control and metal resistant isolates were transferred to plates containing antibiotics. Populations of cobalt, copper, silver, lead and chromium resistant bacteria were similar to the control. Cadmium and mercury resistant populations were significantly lower. Many isolates were resistant to ampicillin, cefamandole, sulfamethoxazole, penicillin, and vancomycin. Most inhibition of growth was seen with ciprofloxacin, doxycycline and rifampin. The results suggest there are much higher levels of metal resistance amongst the river bacterial isolates than expected.

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Metal and antibiotic cross-resistance among bacteria isolated from the Kentucky River

Little is known about metal and antibiotic cross resistance among bacteria in aquatic settings. Water samples were collected from six locations along the Kentucky River. Plate counting was used to quantify metal-resistant bacteria. Control and metal resistant isolates were transferred to plates containing antibiotics. Populations of cobalt, copper, silver, lead and chromium resistant bacteria were similar to the control. Cadmium and mercury resistant populations were significantly lower. Many isolates were resistant to ampicillin, cefamandole, sulfamethoxazole, penicillin, and vancomycin. Most inhibition of growth was seen with ciprofloxacin, doxycycline and rifampin. The results suggest there are much higher levels of metal resistance amongst the river bacterial isolates than expected.