Kentucky State University
Qualitative Analysis of Surrogate Bioremediator Arthrobacter nicotanae
Institution
Kentucky State University
Faculty Advisor/ Mentor
Narayanan Rajendran
Abstract
The growth-supporting ability of culture media and their differences are of deep interest in agro bacteriological and biomedical research. Analysis of microbial growth on culture media is an essential requirement to characterize a bacterium. Serum-based culture media study to achieve a stable bacterial growth is desirable. In the present study, we quantitatively analyzed the growthsupporting property of different media for our newly isolated bioremediator strain of Arthrobacter. For analyses, 1 % of A. nicotanae was inoculated into Nutrient Broth (NB) and the growth was measured at 600nm, using a spectrometer for 48 hours after incubating at 30°C. The OD, pH and temperature were recorded every 4 hours. For Fatal Bovine Serum (FBS) analysis, three sets of NB agar plates with and without 1% and 5% of the FBS were used. For the antimicrobial susceptibility test, 8 types of antibiotic discs (Novobiocin, Penicillin, Tetracycline, Erythromycin, Chloramphenicol, Neomycin, Kanamycin, and Streptomycin) were tested using NB maxi plates. UV analysis was run by exposing the NB plate at specific time intervals with controls. Mueller Hinton Blood agar, Phenol Red Lactose agar, Blood agar and McConkey agar plates were used for selective and differential media analyses. Results indicated that the bacterium had optimal growth in NB. The MH blood agar had good growth, while McMonkey showed no growth. Novobiocin had high effectiveness. Lack of growth was noticed on UV exposed plates. There was no significant difference in the zone of inhibition on FBS plates. The results indicated that these media had different level of growth-inhibiting activity, and suggested that the inhibition was due to the initial decrease in bacterial colonies rather than suppression of the multiplication process during their growth.
Qualitative Analysis of Surrogate Bioremediator Arthrobacter nicotanae
The growth-supporting ability of culture media and their differences are of deep interest in agro bacteriological and biomedical research. Analysis of microbial growth on culture media is an essential requirement to characterize a bacterium. Serum-based culture media study to achieve a stable bacterial growth is desirable. In the present study, we quantitatively analyzed the growthsupporting property of different media for our newly isolated bioremediator strain of Arthrobacter. For analyses, 1 % of A. nicotanae was inoculated into Nutrient Broth (NB) and the growth was measured at 600nm, using a spectrometer for 48 hours after incubating at 30°C. The OD, pH and temperature were recorded every 4 hours. For Fatal Bovine Serum (FBS) analysis, three sets of NB agar plates with and without 1% and 5% of the FBS were used. For the antimicrobial susceptibility test, 8 types of antibiotic discs (Novobiocin, Penicillin, Tetracycline, Erythromycin, Chloramphenicol, Neomycin, Kanamycin, and Streptomycin) were tested using NB maxi plates. UV analysis was run by exposing the NB plate at specific time intervals with controls. Mueller Hinton Blood agar, Phenol Red Lactose agar, Blood agar and McConkey agar plates were used for selective and differential media analyses. Results indicated that the bacterium had optimal growth in NB. The MH blood agar had good growth, while McMonkey showed no growth. Novobiocin had high effectiveness. Lack of growth was noticed on UV exposed plates. There was no significant difference in the zone of inhibition on FBS plates. The results indicated that these media had different level of growth-inhibiting activity, and suggested that the inhibition was due to the initial decrease in bacterial colonies rather than suppression of the multiplication process during their growth.