University of Louisville
Tissue and Gender Differences in Rat Adiponectin Receptor Levels
Institution
University of Louisville
Faculty Advisor/ Mentor
Joseph M. Steffen
Abstract
Obesity and diabetes are both local and international health problems. Adiponectin is a protein secreted from adipocytes and other tissues such as muscle which influences carbohydrate and lipid metabolism. Adiponectin interacts with receptors termed AdipoR1 and AdipoR2, which are similar to G protein-coupled receptors except with a reverse membrane topology. The aim of this study is to determine the levels of AdipoR1 and AdipoR2 within cardiac, skeletal, and liver tissue samples of non-obese rats, with the objective of determining potential differences in the tissue levels of these receptors and determining any gender differences. Samples of tissue were homogenized, the lysate centrifuged to produce a supernatant, and the protein content analyzed. AdipoR1 and AdipoR2 levels were determined by ELISA (NeoBiolab, Boston, MA). The data supports a difference (P≤ 0.02) in AdipoR1 levels between the liver and muscle tissue (gastrocnemius, soleus, and heart), as well as between the genders for the gastrocnemius and liver. The levels of AdipoR2 in the liver were also significantly lower than in muscles (heart and gastrocnemius), with significant differences (P≤ 0.01) between the genders for liver. The data from the current study supports previous work on the relative levels of AdipoR1 between muscles and liver, but does not identify any difference between muscle types. In addition, gender differences in the levels of AdipoR1 in gastrocnemius and liver are novel observations. However, the data from the current study contradicts that of previous reports, which had suggested that liver had a higher level of AdipoR2 than muscle tissue, indicating that further investigation and confirmation is necessary.
Tissue and Gender Differences in Rat Adiponectin Receptor Levels
Obesity and diabetes are both local and international health problems. Adiponectin is a protein secreted from adipocytes and other tissues such as muscle which influences carbohydrate and lipid metabolism. Adiponectin interacts with receptors termed AdipoR1 and AdipoR2, which are similar to G protein-coupled receptors except with a reverse membrane topology. The aim of this study is to determine the levels of AdipoR1 and AdipoR2 within cardiac, skeletal, and liver tissue samples of non-obese rats, with the objective of determining potential differences in the tissue levels of these receptors and determining any gender differences. Samples of tissue were homogenized, the lysate centrifuged to produce a supernatant, and the protein content analyzed. AdipoR1 and AdipoR2 levels were determined by ELISA (NeoBiolab, Boston, MA). The data supports a difference (P≤ 0.02) in AdipoR1 levels between the liver and muscle tissue (gastrocnemius, soleus, and heart), as well as between the genders for the gastrocnemius and liver. The levels of AdipoR2 in the liver were also significantly lower than in muscles (heart and gastrocnemius), with significant differences (P≤ 0.01) between the genders for liver. The data from the current study supports previous work on the relative levels of AdipoR1 between muscles and liver, but does not identify any difference between muscle types. In addition, gender differences in the levels of AdipoR1 in gastrocnemius and liver are novel observations. However, the data from the current study contradicts that of previous reports, which had suggested that liver had a higher level of AdipoR2 than muscle tissue, indicating that further investigation and confirmation is necessary.